Error analysis of idealized nanopore sequencing
نویسندگان
چکیده
منابع مشابه
Error analysis of idealized nanopore sequencing.
This numerical study provides an error analysis of an idealized nanopore sequencing method in which ionic current measurements are used to sequence intact single-stranded DNA in the pore, while an enzyme controls DNA motion. Examples of systematic channel errors when more than one nucleotide affects the current amplitude are detailed, which if present will persist regardless of coverage. Absent...
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Received 6 January 2015; accepted 5 November 2015; published online 6 May 2016; doi:10.1038/nbt.3423 and together with Sergey Bezrukov at the US National Institutes of Health (NIH), had established the conditions that were necessary to avoid spontaneous gating (pore closure) of the a-hemolysin channel3. This was important, because spontaneous gating would have hindered, or confused, observation...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2013
ISSN: 0173-0835
DOI: 10.1002/elps.201300174